Intracardiac electrical stimulation

To determine the susceptibility to pacing-induced arrhythmias and conduction property by simultaneous recording of surface ECG and intracardiac electrograms.

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Optical Mapping
Optical imaging of the perfused heart to determine the activation pattern visually.
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Left:   normal activation pattern


Right: an ectopic firing near the lower right atrium lead to a              premature p'-wave on ECG

Live cell imaging

Confocal imaging of freshly isolated cardiomyocytes with fluorescent indicators.

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Red arrow points to the spontaneous Ca2+ release event (Ca2+ wave)

Detect the subcellular localization of the protein of interest in adult cardiomyocytes
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Dual staining of the myocyte marker (a-actinin) and IL-1R in an atrial myocyte.

AAV-mediated gene transfer

Modify cardiomyocyte gene expression using a variety of AAV9 vectors.

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"Flox-stop-shRNA" system to induce NLRP3 knockdown in cardiomyocyte specifically (Yao et al. Circulation. 2018)